An outbreak of novel coronavirus (2019-nCoV) was initially reported in Wuhan City, Hubei Province, China on December 31, 2019. Chinese health authorities have now confirmed thousands of infections and over 100 deaths. On January 30th, 2020, the International Health Regulations Emergency Committee of the World Health Organization declare the outbreak a “public health emergency of international concern” (PHEIC). The first case in the United States (US) was identified in Washington state on January 21, 2020. As of January 31, 2020, a total of six positive cases have been reported in the US; five are travel-associated and one is local transmission in the US. On January 31, 2020, the Centers for Disease Control and Prevention (CDC) updated the case definition for patients under investigation (PUI). This Health Notice provides updated guidance for evaluation of PUIs for 2019-nCoV, prevention and infection control guidance, and information on specimen collection.